En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit D Dimer ELISA kit (E04D0224)

E04D0224 Rabbit D Dimer ELISA kit

Rabbit D Dimer ELISA kit is suitable for detecting samples from rabbit species. D Dimer can also be called D2D, D 2 Dimer, D-Dimer.

Products

Rabbit D Dimer ELISA kit

Product Information

Cat. No.

E04D0224

Product Name

Rabbit D Dimer ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ug/mL

Sensitivity

0.1 ug/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ug/mL

1 vial

STANDARD B (0.5mL)

0.5 ug/mL

1 vial

STANDARD C (0.5mL)

1.0 ug/mL

1 vial

STANDARD D (0.5mL)

2.5 ug/mL

1 vial

STANDARD E (0.5mL)

5.0 ug/mL

1 vial

STANDARD F (0.5mL)

10 ug/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

D2D ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- D2D antibody and an D2D-HRP conjugate. The assay sample and buffer are incubated together with D2D-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the D2D concentration since D2D from samples and D2D-HRP conjugate compete for the anti-D2D antibody binding site. Since the number of sites is limited, as more sites are occupied by D2D from the sample, fewer sites are left to bind D2D-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The D2D concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit D Dimer ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between D2D and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit D Dimer ELISA kit

Summary of the Assay Procedure for Rabbit D Dimer ELISA kit

Citations of Rabbit D Dimer ELISA kit

E04D0224 has been referenced in the below publications:

The Study about Mechanism of Onion stalk extraction on Prevention and Treatment of Rabbits’Traumatic Deep Vein Thrombosis.

化瘀解毒法对急性肺血栓栓塞症兔D-二聚体、内皮素-1、B型脑利钠肽表达影响.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.