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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit Endothelial Nitric Oxide Synthase ELISA kit (E04N0024)

E04N0024 Rabbit Endothelial Nitric Oxide Synthase ELISA kit

Rabbit Endothelial Nitric Oxide Synthase ELISA kit is suitable for detecting samples from rabbit species. Endothelial Nitric Oxide Synthase can also be called NOS-3, cNOS, Constitutive NOS, ecNOS, Endothelial nitric oxidase synthase, Endothelial nitric oxide synthase 3, Endothelial NOS, Nitric oxide synthase 3 (endothelial cell), Nitric oxide synthase 3, Nitric oxide synthase 3 endothelial cell, Nitric oxide synthase endothelial, nitric oxide synthase, endothelial, NOS 3, NOS III, NOS type III, eNOS.

Products

Specifications of Rabbit Endothelial Nitric Oxide Synthase ELISA kit

Product Information

Cat. No.

E04N0024

Product Name

Rabbit Endothelial Nitric Oxide Synthase ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

eNOS ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- eNOS antibody and an eNOS-HRP conjugate. The assay sample and buffer are incubated together with eNOS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the eNOS concentration since eNOS from samples and eNOS-HRP conjugate compete for the anti-eNOS antibody binding site. Since the number of sites is limited, as more sites are occupied by eNOS from the sample, fewer sites are left to bind eNOS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The eNOS concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit Endothelial Nitric Oxide Synthase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between eNOS and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit Endothelial Nitric Oxide Synthase ELISA kit

Summary of the Assay Procedure for Rabbit Endothelial Nitric Oxide Synthase ELISA kit

Citations of Rabbit Endothelial Nitric Oxide Synthase ELISA kit

E04N0024 has been referenced in the below publications:

To explore the inhibition of Polyinosinic acid combined statin on Cholesterol crystals as well as expression of hs-crp in atherosclerotic rabbits.

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