Name: Rabbit Endothelin 1 ELISA kit (E04E0040)
Brand: Shanghai BlueGene Biotech CO., LTD.
Price: 100 USD

BlueGene Biotech Rabbit Endothelin 1 ELISA kit (E04E0040)

E04E0040 Rabbit Endothelin 1 ELISA kit

Rabbit Endothelin 1 ELISA kit is suitable for detecting samples from rabbit species. Endothelin 1 can also be called Endothelin-1, Big endothelin 1, EDN1 protein, Endothelin1, ET1, PPET1, Preproendothelin 1, EDN1, EDN 1, EDN-1.

Products

Rabbit Endothelin 1 ELISA kit

Product Information
Cat. No.	E04E0040
Product Name	Rabbit Endothelin 1 ELISA kit
Species	Rabbit
Product Size	48 Tests / 96 Tests
Concentration	0.5-10ng/mL
Sensitivity	0.1 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	0.5 ng/mL	1 vial
STANDARD C (0.5mL)	1.0 ng/mL	1 vial
STANDARD D (0.5mL)	2.5 ng/mL	1 vial
STANDARD E (0.5mL)	5.0 ng/mL	1 vial
STANDARD F (0.5mL)	10 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

EDN1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- EDN1 antibody and an EDN1-HRP conjugate. The assay sample and buffer are incubated together with EDN1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EDN1 concentration since EDN1 from samples and EDN1-HRP conjugate compete for the anti-EDN1 antibody binding site. Since the number of sites is limited, as more sites are occupied by EDN1 from the sample, fewer sites are left to bind EDN1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EDN1 concentration in each sample is interpolated from this standard curve.

Quality Control on Rabbit Endothelin 1 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	89-114.79%
Linearity	Diluent Ratio	Range %
	1:2	96-116
	1:4	91-109
	1:8	88-103
Specificity/Cross-reactivity	No significant cross-reactivity or interference between EDN1 and analogues was observed.