E04F0007 Rabbit Fibrinogen ELISA kit
Rabbit Fibrinogen ELISA kit is suitable for detecting samples from rabbit species. Fibrinogen can also be called F1, FI, Fbg, Coagulation Factor I, Fb.
Specifications of Rabbit Fibrinogen ELISA kit
Product Information | |
Cat. No. | E04F0007 |
Product Name | Rabbit Fibrinogen ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10mg/mL |
Sensitivity | 0.1 mg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 mg/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 mg/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 mg/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 mg/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 mg/mL | 1 vial |
STANDARD F (0.5mL) | 10 mg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Fb ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- Fb antibody and an Fb-HRP conjugate. The assay sample and buffer are incubated together with Fb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Fb concentration since Fb from samples and Fb-HRP conjugate compete for the anti-Fb antibody binding site. Since the number of sites is limited, as more sites are occupied by Fb from the sample, fewer sites are left to bind Fb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Fb concentration in each sample is interpolated from this standard curve. |
Quality Control on Rabbit Fibrinogen ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-114.79% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-116 | |
1:4 | 91-109 | |
1:8 | 88-103 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Fb and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rabbit Fibrinogen ELISA kit
Citations of Rabbit Fibrinogen ELISA kit
E04F0007 has been referenced in the below publications:
Modulation of osteogenic and haemostatic activities by tuning cationicity of genipin-crosslinked chitosan hydrogels.
