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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit Heart Fatty Acid Binding Protein ELISA kit (E04H0124)

E04H0124 Rabbit Heart Fatty Acid Binding Protein ELISA kit

Rabbit Heart Fatty Acid Binding Protein ELISA kit is suitable for detecting samples from rabbit species. Heart Fatty Acid Binding Protein can also be called hFABP, H-FABP, FABP3, FABP11, M-FABP, MDGI, O-FABP, Heart-type fatty acid binding protein, fatty acid binding protein 3, mammary-derived growth inhibitor, h FABP.

Products

Specifications of Rabbit Heart Fatty Acid Binding Protein ELISA kit

Product Information

Cat. No.

E04H0124

Product Name

Rabbit Heart Fatty Acid Binding Protein ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

5.0-100ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

h FABP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- h FABP antibody and an h FABP-HRP conjugate. The assay sample and buffer are incubated together with h FABP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the h FABP concentration since h FABP from samples and h FABP-HRP conjugate compete for the anti-h FABP antibody binding site. Since the number of sites is limited, as more sites are occupied by h FABP from the sample, fewer sites are left to bind h FABP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The h FABP concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit Heart Fatty Acid Binding Protein ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between h FABP and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit Heart Fatty Acid Binding Protein ELISA kit

Summary of the Assay Procedure for Rabbit Heart Fatty Acid Binding Protein ELISA kit

Citations of Rabbit Heart Fatty Acid Binding Protein ELISA kit

E04H0124 has been referenced in the below publications:

Serum H-FABP Content Changes After Myocardial Ischemia And Intervention Study Of Guizhi Soup Compound Prescription In New Zealand Rabbits.

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