E04H0094 Rabbit Heme Oxygenase 1, Decycling ELISA kit
Rabbit Heme Oxygenase 1, Decycling ELISA kit is suitable for detecting samples from rabbit species. Heme Oxygenase 1, Decycling can also be called HO1, HO-1, HSP32, bK286B10, HMOX1D, HMOX1, HSP32, Heme oxygenase (decycling) 1, Heme oxygenase 1, Hemox, HMOX 1, Hmox, HO 1, Hsp32, bK286B10, heme oxygenase 1.
Product Information | |
Cat. No. | E04H0094 |
Product Name | Rabbit Heme Oxygenase 1, Decycling ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
HO1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- HO1 antibody and an HO1-HRP conjugate. The assay sample and buffer are incubated together with HO1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HO1 concentration since HO1 from samples and HO1-HRP conjugate compete for the anti-HO1 antibody binding site. Since the number of sites is limited, as more sites are occupied by HO1 from the sample, fewer sites are left to bind HO1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HO1 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-114.79% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-116 | |
1:4 | 91-109 | |
1:8 | 88-103 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between HO1 and analogues was observed. | |
E04H0094 has been referenced in the below publications:
The Nrf2-ARE Pathway Is Associated With Schisandrin B Attenuating Benzo(a)pyrene_x005fInduced HTR Cells Damages in Vitro.
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