Name: Rabbit Heme Oxygenase 1, Decycling ELISA kit (E04H0094)
Brand: Shanghai BlueGene Biotech CO., LTD.
Price: 100 USD

BlueGene Biotech Rabbit Heme Oxygenase 1, Decycling ELISA kit (E04H0094)

E04H0094 Rabbit Heme Oxygenase 1, Decycling ELISA kit

Rabbit Heme Oxygenase 1, Decycling ELISA kit is suitable for detecting samples from rabbit species. Heme Oxygenase 1, Decycling can also be called HO1, HO-1, HSP32, bK286B10, HMOX1D, HMOX1, HSP32, Heme oxygenase (decycling) 1, Heme oxygenase 1, Hemox, HMOX 1, Hmox, HO 1, Hsp32, bK286B10, heme oxygenase 1.

Products

Specifications of Rabbit Heme Oxygenase 1, Decycling ELISA kit

Product Information
Cat. No.	E04H0094
Product Name	Rabbit Heme Oxygenase 1, Decycling ELISA kit
Species	Rabbit
Product Size	48 Tests / 96 Tests
Concentration	1.0-25ng/mL
Sensitivity	0.1 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	1.0 ng/mL	1 vial
STANDARD C (0.5mL)	2.5 ng/mL	1 vial
STANDARD D (0.5mL)	5.0 ng/mL	1 vial
STANDARD E (0.5mL)	10 ng/mL	1 vial
STANDARD F (0.5mL)	25 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

HO1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- HO1 antibody and an HO1-HRP conjugate. The assay sample and buffer are incubated together with HO1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HO1 concentration since HO1 from samples and HO1-HRP conjugate compete for the anti-HO1 antibody binding site. Since the number of sites is limited, as more sites are occupied by HO1 from the sample, fewer sites are left to bind HO1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HO1 concentration in each sample is interpolated from this standard curve.

Quality Control on Rabbit Heme Oxygenase 1, Decycling ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	89-114.79%
Linearity	Diluent Ratio	Range %
	1:2	96-116
	1:4	91-109
	1:8	88-103
Specificity/Cross-reactivity	No significant cross-reactivity or interference between HO1 and analogues was observed.