E04H0208 Rabbit Hepatocyte Growth Factor ELISA kit
Rabbit Hepatocyte Growth Factor ELISA kit is suitable for detecting samples from rabbit species. Hepatocyte Growth Factor can also be called HGF, HGF alpha, HGFA, F TCF, Hepatocyte growth factor, Fibroblast derived tumor cytotoxic factor, Hepatocyte growth factor precursor, Hepatopoietin A, HPTA, HGF.
Product Information | |
Cat. No. | E04H0208 |
Product Name | Rabbit Hepatocyte Growth Factor ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
HGF ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- HGF antibody and an HGF-HRP conjugate. The assay sample and buffer are incubated together with HGF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HGF concentration since HGF from samples and HGF-HRP conjugate compete for the anti-HGF antibody binding site. Since the number of sites is limited, as more sites are occupied by HGF from the sample, fewer sites are left to bind HGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HGF concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-114.79% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-116 | |
1:4 | 91-109 | |
1:8 | 88-103 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between HGF and analogues was observed. | |
E04H0208 has been referenced in the below publications:
Harvesting prevascularized smooth muscle cell sheets from common polystyrene culture dishes.
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