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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit Intact Parathormone ELISA kit (E04P0572)

E04P0572 Rabbit Intact Parathormone ELISA kit

Rabbit Intact Parathormone ELISA kit is suitable for detecting samples from rabbit species. Intact Parathormone can also be called PTH, PTH1, parathyroid hormone, Parathyroid hormone, FIH1, parathormone, parathyrin, I PTH.

Products

Specifications of Rabbit Intact Parathormone ELISA kit

Product Information

Cat. No.

E04P0572

Product Name

Rabbit Intact Parathormone ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

I PTH ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- I PTH antibody and an I PTH-HRP conjugate. The assay sample and buffer are incubated together with I PTH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the I PTH concentration since I PTH from samples and I PTH-HRP conjugate compete for the anti-I PTH antibody binding site. Since the number of sites is limited, as more sites are occupied by I PTH from the sample, fewer sites are left to bind I PTH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The I PTH concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit Intact Parathormone ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between I PTH and analogues was observed.


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Summary of the Assay Procedure for Rabbit Intact Parathormone ELISA kit

Summary of the Assay Procedure for Rabbit Intact Parathormone ELISA kit

Citations of Rabbit Intact Parathormone ELISA kit

E04P0572 has been referenced in the below publications:

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