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  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit Matrix metalloproteinase 2 ELISA kit (E04M0304)

E04M0304 Rabbit Matrix metalloproteinase 2 ELISA kit

Rabbit Matrix metalloproteinase 2 ELISA kit is suitable for detecting samples from rabbit species. Matrix metalloproteinase 2 can also be called 72 kDa gelatinase, 72kD type IV collagenase, CLG 4, CLG 4A, Collagenase Type 4 alpha, Collagenase type IV A, Gelatinase A, Gelatinase alpha, Gelatinase neutrophil, Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase, Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase), Matrix Metalloproteinase 2, Matrix metalloproteinase II, MMP II, MONA, Neutrophil gelatinase, TBE 1, MMP 2, MMP2, MMP-2.

Products

Specifications of Rabbit Matrix metalloproteinase 2 ELISA kit

Product Information

Cat. No.

E04M0304

Product Name

Rabbit Matrix metalloproteinase 2 ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

2.5-50ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

MMP 2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- MMP 2 antibody and an MMP 2-HRP conjugate. The assay sample and buffer are incubated together with MMP 2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MMP 2 concentration since MMP 2 from samples and MMP 2-HRP conjugate compete for the anti-MMP 2 antibody binding site. Since the number of sites is limited, as more sites are occupied by MMP 2 from the sample, fewer sites are left to bind MMP 2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP 2 concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit Matrix metalloproteinase 2 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between MMP 2 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit Matrix metalloproteinase 2 ELISA kit

Summary of the Assay Procedure for Rabbit Matrix metalloproteinase 2 ELISA kit

Citations of Rabbit Matrix metalloproteinase 2 ELISA kit

E04M0304 has been referenced in the below publications:

The study of stabilizing atherosclerotic vulnerable plaque by gene silencing CD147 using lentiviral-delivered small interference RNA.

Effect of rosuvatation on the stability of the plaque and expression of CD147 in atherosclerotic plaques of rabbits.

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