E04M0329 Rabbit Matrix metalloproteinase 9 ELISA kit
Rabbit Matrix metalloproteinase 9 ELISA kit is suitable for detecting samples from rabbit species. Matrix metalloproteinase 9 can also be called Matrix metalloproteinase-9 precursor, 92 kDa type IV. Collagenase, 92 kDa gelatinase, Gelatinase B, GELB, MMP 9, MMP9, MMP-9.
Product Information | |
Cat. No. | E04M0329 |
Product Name | Rabbit Matrix metalloproteinase 9 ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
MMP 9 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- MMP 9 antibody and an MMP 9-HRP conjugate. The assay sample and buffer are incubated together with MMP 9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MMP 9 concentration since MMP 9 from samples and MMP 9-HRP conjugate compete for the anti-MMP 9 antibody binding site. Since the number of sites is limited, as more sites are occupied by MMP 9 from the sample, fewer sites are left to bind MMP 9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP 9 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-114.79% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-116 | |
1:4 | 91-109 | |
1:8 | 88-103 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between MMP 9 and analogues was observed. | |
E04M0329 has been referenced in the below publications:
The study of stabilizing atherosclerotic vulnerable plaque by gene silencing CD147 using lentiviral-delivered small interference RNA.
Effect of rosuvatation on the stability of the plaque and expression of CD147 in atherosclerotic plaques of rabbits.
To explore the inhibition of Polyinosinic acid combined statin on Cholesterol crystals as well as expression of hs-crp in atherosclerotic rabbits.
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