Name: Rabbit N Acetylgalactosamine 6 Sulfatase ELISA kit (E04G0081)
Brand: Shanghai BlueGene Biotech CO., LTD.
Price: 100 USD

BlueGene Biotech Rabbit N Acetylgalactosamine 6 Sulfatase ELISA kit (E04G0081)

E04G0081 Rabbit N Acetylgalactosamine 6 Sulfatase ELISA kit

Rabbit N Acetylgalactosamine 6 Sulfatase ELISA kit is suitable for detecting samples from rabbit species. N Acetylgalactosamine 6 Sulfatase can also be called Chondroitinase, Chondroitinsulfatase, FLJ00319, FLJ17434, FLJ42844, FLJ98217, Galactosamine (N acetyl) 6 sulfate sulfatase, Galactose 6 sulfate sulfatase, GALNAC6S, GalNAc6S sulfatase, GAS, mFLJ00319, MPS4A, N acetylgalactosamine 6 sulfatase, N acetylgalactosamine 6 sulfate sulfatase, GAS.

Products

Specifications of Rabbit N Acetylgalactosamine 6 Sulfatase ELISA kit

Product Information
Cat. No.	E04G0081
Product Name	Rabbit N Acetylgalactosamine 6 Sulfatase ELISA kit
Species	Rabbit
Product Size	48 Tests / 96 Tests
Concentration	10-250ng/mL
Sensitivity	1.0 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	10 ng/mL	1 vial
STANDARD C (0.5mL)	25 ng/mL	1 vial
STANDARD D (0.5mL)	50 ng/mL	1 vial
STANDARD E (0.5mL)	100 ng/mL	1 vial
STANDARD F (0.5mL)	250 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

GAS ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- GAS antibody and an GAS-HRP conjugate. The assay sample and buffer are incubated together with GAS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GAS concentration since GAS from samples and GAS-HRP conjugate compete for the anti-GAS antibody binding site. Since the number of sites is limited, as more sites are occupied by GAS from the sample, fewer sites are left to bind GAS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GAS concentration in each sample is interpolated from this standard curve.

Quality Control on Rabbit N Acetylgalactosamine 6 Sulfatase ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	89-114.79%
Linearity	Diluent Ratio	Range %
	1:2	96-116
	1:4	91-109
	1:8	88-103
Specificity/Cross-reactivity	No significant cross-reactivity or interference between GAS and analogues was observed.