En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit N Acetylgalactosamine 6 Sulfatase ELISA kit (E04G0081)

E04G0081 Rabbit N Acetylgalactosamine 6 Sulfatase ELISA kit

Rabbit N Acetylgalactosamine 6 Sulfatase ELISA kit is suitable for detecting samples from rabbit species. N Acetylgalactosamine 6 Sulfatase can also be called Chondroitinase, Chondroitinsulfatase, FLJ00319, FLJ17434, FLJ42844, FLJ98217, Galactosamine (N acetyl) 6 sulfate sulfatase, Galactose 6 sulfate sulfatase, GALNAC6S, GalNAc6S sulfatase, GAS, mFLJ00319, MPS4A, N acetylgalactosamine 6 sulfatase, N acetylgalactosamine 6 sulfate sulfatase, GAS.

Products

Specifications of Rabbit N Acetylgalactosamine 6 Sulfatase ELISA kit

Product Information

Cat. No.

E04G0081

Product Name

Rabbit N Acetylgalactosamine 6 Sulfatase ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

10-250ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

10 ng/mL

1 vial

STANDARD C (0.5mL)

25 ng/mL

1 vial

STANDARD D (0.5mL)

50 ng/mL

1 vial

STANDARD E (0.5mL)

100 ng/mL

1 vial

STANDARD F (0.5mL)

250 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

GAS ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- GAS antibody and an GAS-HRP conjugate. The assay sample and buffer are incubated together with GAS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GAS concentration since GAS from samples and GAS-HRP conjugate compete for the anti-GAS antibody binding site. Since the number of sites is limited, as more sites are occupied by GAS from the sample, fewer sites are left to bind GAS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GAS concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit N Acetylgalactosamine 6 Sulfatase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between GAS and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit N Acetylgalactosamine 6 Sulfatase ELISA kit

Summary of the Assay Procedure for Rabbit N Acetylgalactosamine 6 Sulfatase ELISA kit

Citations of Rabbit N Acetylgalactosamine 6 Sulfatase ELISA kit

E04T0229 has been referenced in the below publications:

Effect of Banxia Houpu Decoction on EGF and GasConcentration in Peripheral Blood of Rabbits with Vomit Caused By Chemotherapy.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.