E04N0014 Rabbit Nerve Growth Factor ELISA kit
Rabbit Nerve Growth Factor ELISA kit is suitable for detecting samples from rabbit species. Nerve Growth Factor can also be called NGFB, Beta-NGF, HSAN5, NGF-B, Beta-Nerve Growth Factor, NGF.
Specifications of Rabbit Nerve Growth Factor ELISA kit
Product Information | |
Cat. No. | E04N0014 |
Product Name | Rabbit Nerve Growth Factor ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
NGF ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- NGF antibody and an NGF-HRP conjugate. The assay sample and buffer are incubated together with NGF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NGF concentration since NGF from samples and NGF-HRP conjugate compete for the anti-NGF antibody binding site. Since the number of sites is limited, as more sites are occupied by NGF from the sample, fewer sites are left to bind NGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NGF concentration in each sample is interpolated from this standard curve. |
Quality Control on Rabbit Nerve Growth Factor ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-114.79% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-116 | |
1:4 | 91-109 | |
1:8 | 88-103 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between NGF and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rabbit Nerve Growth Factor ELISA kit
Citations of Rabbit Nerve Growth Factor ELISA kit
E04N0014 has been referenced in the below publications:
Experiment study on the committed differentiation of adipose-derived stem cells in vitro and used into tissue-engineered peripheral nerves.
