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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit Nitrotyrosine ELISA kit (E04N0005)

E04N0005 Rabbit Nitrotyrosine ELISA kit

Rabbit Nitrotyrosine ELISA kit is suitable for detecting samples from rabbit species. Nitrotyrosine can also be called 3-Nitro-L-Tyrosine, 3-Nitrotyrosine, NT.

Products

Specifications of Rabbit Nitrotyrosine ELISA kit

Product Information

Cat. No.

E04N0005

Product Name

Rabbit Nitrotyrosine ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

5.0-100ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

NT ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- NT antibody and an NT-HRP conjugate. The assay sample and buffer are incubated together with NT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NT concentration since NT from samples and NT-HRP conjugate compete for the anti-NT antibody binding site. Since the number of sites is limited, as more sites are occupied by NT from the sample, fewer sites are left to bind NT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NT concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit Nitrotyrosine ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between NT and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit Nitrotyrosine ELISA kit

Summary of the Assay Procedure for Rabbit Nitrotyrosine ELISA kit

Citations of Rabbit Nitrotyrosine ELISA kit

E04N0005 has been referenced in the below publications:

N-acetylcysteine advancement of surfactant therapy in experimental meconium aspiration syndrome: possible mechanisms.

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