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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit Nuclear factor kB ELISA kit (E04N0011)

E04N0011 Rabbit Nuclear factor kB ELISA kit

Rabbit Nuclear factor kB ELISA kit is suitable for detecting samples from rabbit species. Nuclear factor kB can also be called NF-KB1, EBP-1, KBF1, NF-Kappa-B, NFKB-P105, NFKB-P50, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1, Nuclear factor NF-kappa-B p105 subunit, NFkB.

Products

Specifications of Rabbit Nuclear factor kB ELISA kit

Product Information

Cat. No.

E04N0011

Product Name

Rabbit Nuclear factor kB ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

5.0-100ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

NFkB ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- NFkB antibody and an NFkB-HRP conjugate. The assay sample and buffer are incubated together with NFkB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NFkB concentration since NFkB from samples and NFkB-HRP conjugate compete for the anti-NFkB antibody binding site. Since the number of sites is limited, as more sites are occupied by NFkB from the sample, fewer sites are left to bind NFkB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NFkB concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit Nuclear factor kB ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between NFkB and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit Nuclear factor kB ELISA kit

Summary of the Assay Procedure for Rabbit Nuclear factor kB ELISA kit

Citations of Rabbit Nuclear factor kB ELISA kit

E04N0011 has been referenced in the below publications:

Effect of ischemic postconditioning on NF-kB activation signaling pathway in early lung transplantation.

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