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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit Osteocalcin ELISA kit (E04O0001)

E04O0001 Rabbit Osteocalcin ELISA kit

Rabbit Osteocalcin ELISA kit is suitable for detecting samples from rabbit species. Osteocalcin can also be called Osteocalcin Propeptide, Osteocalcin, Osteocalcin precursor, BGLAP, Bone gamma carboxyglutamate protein, BGP, Bone gamma carboxyglutamate gla protein osteocalcin, Bone Gla protein, Gamma carboxyglutamic acid containing protein, OC.

Products

Specifications of Rabbit Osteocalcin ELISA kit

Product Information

Cat. No.

E04O0001

Product Name

Rabbit Osteocalcin ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

2.5-50ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

OC ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- OC antibody and an OC-HRP conjugate. The assay sample and buffer are incubated together with OC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OC concentration since OC from samples and OC-HRP conjugate compete for the anti-OC antibody binding site. Since the number of sites is limited, as more sites are occupied by OC from the sample, fewer sites are left to bind OC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OC concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit Osteocalcin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between OC and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit Osteocalcin ELISA kit

Summary of the Assay Procedure for Rabbit Osteocalcin ELISA kit

Citations of Rabbit Osteocalcin ELISA kit

E04O0001 has been referenced in the below publications:

Modulation of osteogenic and haemostatic activities by tuning cationicity of genipin-crosslinked chitosan hydrogels.

Rational design of gelatin/nanohydroxyapatite cryogel scaffolds for bone regeneration by introducing chemical and physical cues to enhance osteogenesis of bone marrow mesenchymal stem cells.

Investigation of synergistic effects of inductive and conductive factors in gelatin-based cryogels for bone tissue engineering.

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