E04O0341 Rabbit Oxidized lowdensity lipoprotein ELISA kit
Rabbit Oxidized lowdensity lipoprotein ELISA kit is suitable for detecting samples from rabbit species. Oxidized lowdensity lipoprotein can also be called OL, OxLDL.
Rabbit Oxidized lowdensity lipoprotein ELISA kit
Product Information | |
Cat. No. | E04O0341 |
Product Name | Rabbit Oxidized lowdensity lipoprotein ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
OxLDL ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- OxLDL antibody and an OxLDL-HRP conjugate. The assay sample and buffer are incubated together with OxLDL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OxLDL concentration since OxLDL from samples and OxLDL-HRP conjugate compete for the anti-OxLDL antibody binding site. Since the number of sites is limited, as more sites are occupied by OxLDL from the sample, fewer sites are left to bind OxLDL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OxLDL concentration in each sample is interpolated from this standard curve. |
Quality Control on Rabbit Oxidized lowdensity lipoprotein ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-114.79% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-116 | |
1:4 | 91-109 | |
1:8 | 88-103 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between OxLDL and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rabbit Oxidized lowdensity lipoprotein ELISA kit
Citations of Rabbit Oxidized lowdensity lipoprotein ELISA kit
E04O0341 has been referenced in the below publications:
To explore the inhibition of Polyinosinic acid combined statin on Cholesterol crystals as well as expression of hs-crp in atherosclerotic rabbits.
额尔敦-乌日勒对动脉粥样硬化兔血清ox-LDL含量及主动脉LOX-1表达的影响.
