E04P0018 Rabbit Placenta Growth Factor ELISA kit
Rabbit Placenta Growth Factor ELISA kit is suitable for the detection of samples from Rabbit species. Placenta Growth Factor can also be called PlGF2, PGF, PGFL, Placental Growth Factor-Like, Vascular Endothelial Growth Factor-Related Protein, PLGF.
Product Information | |
Cat. No. | E04P0018 |
Product Name | Rabbit Placenta Growth Factor ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PLGF ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PLGF. Standards or samples are then added to the microtiter plate wells and PLGF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PLGF present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PLGF are added to each well to “sandwich” the PLGF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PLGF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLGF concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 86-115% | |
Linearity | Diluent Ratio | Range % |
1:2 | 84-114 | |
1:4 | 87-117 | |
1:8 | 80-112 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PLGF and analogues was observed. | |
E04P0018 has been referenced in the below publications:
Inhibition of placental growth factor improves surgical outcome of glaucoma surgery.
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