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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit Serum Albumin ELISA kit (E04S0014)

E04S0014 Rabbit Serum Albumin ELISA kit

Rabbit Serum Albumin ELISA kit is suitable for detecting samples from rabbit species. Serum Albumin can also be called Bovine Albumin, Fraction V, SALB.

Products

Specifications of Rabbit Serum Albumin ELISA kit

Product Information

Cat. No.

E04S0014

Product Name

Rabbit Serum Albumin ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

50-1000ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

50 ng/mL

1 vial

STANDARD C (0.5mL)

100 ng/mL

1 vial

STANDARD D (0.5mL)

250 ng/mL

1 vial

STANDARD E (0.5mL)

500 ng/mL

1 vial

STANDARD F (0.5mL)

1000 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SALB ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- SALB antibody and an SALB-HRP conjugate. The assay sample and buffer are incubated together with SALB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SALB concentration since SALB from samples and SALB-HRP conjugate compete for the anti-SALB antibody binding site. Since the number of sites is limited, as more sites are occupied by SALB from the sample, fewer sites are left to bind SALB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SALB concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit Serum Albumin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SALB and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit Serum Albumin ELISA kit

Summary of the Assay Procedure for Rabbit Serum Albumin ELISA kit

Citations of Rabbit Serum Albumin ELISA kit

E04S0014 has been referenced in the below publications:

Study on the relationship of notochord cell and intervertebral disc degeneration.

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