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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit Tissue type Plasminogen Activator ELISA kit (E04T0010)

E04T0010 Rabbit Tissue type Plasminogen Activator ELISA kit

Rabbit Tissue type Plasminogen Activator ELISA kit is suitable for the detection of samples from Rabbit species. Tissue type Plasminogen Activator can also be called PLAT, T-PA, TPA, plasminogen activator, tissue type, tPA.

Products

Specifications of Rabbit Tissue type Plasminogen Activator ELISA kit

Product Information

Cat. No.

E04T0010

Product Name

Rabbit Tissue type Plasminogen Activator ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

100-2500 pg/mL

Sensitivity

1.0 pg/mL

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

100 pg/mL

1 vial

STANDARD C (0.5mL)

250 pg/mL

1 vial

STANDARD D (0.5mL)

500 pg/mL

1 vial

STANDARD E (0.5mL)

1000 pg/mL

1 vial

STANDARD F (0.5mL)

2500 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

tPA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for tPA. Standards or samples are then added to the microtiter plate wells and tPA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of tPA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for tPA are added to each well to “sandwich” the tPA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain tPA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The tPA concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit Tissue type Plasminogen Activator ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

86-115%

Linearity

Diluent Ratio

Range %

1:2

84-114

1:4

87-117

1:8

80-112

Specificity/Cross-reactivity

No significant cross-reactivity or interference between tPA and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit Tissue type Plasminogen Activator ELISA kit

Summary of the Assay Procedure for Rabbit Tissue type Plasminogen Activator ELISA kit

Citations of Rabbit Tissue type Plasminogen Activator ELISA kit

E04T0010 has been referenced in the below publications:

The Study about Mechanism of Onion stalk extraction on Prevention and Treatment of Rabbits’Traumatic Deep Vein Thrombosis.

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