E04T0010 Rabbit Tissue type Plasminogen Activator ELISA kit
Rabbit Tissue type Plasminogen Activator ELISA kit is suitable for the detection of samples from Rabbit species. Tissue type Plasminogen Activator can also be called PLAT, T-PA, TPA, plasminogen activator, tissue type, tPA.
Product Information | |
Cat. No. | E04T0010 |
Product Name | Rabbit Tissue type Plasminogen Activator ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
tPA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for tPA. Standards or samples are then added to the microtiter plate wells and tPA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of tPA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for tPA are added to each well to “sandwich” the tPA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain tPA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The tPA concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 86-115% | |
Linearity | Diluent Ratio | Range % |
1:2 | 84-114 | |
1:4 | 87-117 | |
1:8 | 80-112 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between tPA and analogues was observed. | |
E04T0010 has been referenced in the below publications:
The Study about Mechanism of Onion stalk extraction on Prevention and Treatment of Rabbits’Traumatic Deep Vein Thrombosis.
Related Bluegene Biotech Products
BlueGene Biotech News
