En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rabbit Total Endothelin ELISA kit (E04E0182)

E04E0182 Rabbit Total Endothelin ELISA kit

Rabbit Total Endothelin ELISA kit is suitable for detecting samples from rabbit species. Total Endothelin can also be called ET.

Products

Specifications of Rabbit Total Endothelin ELISA kit

Product Information

Cat. No.

E04E0182

Product Name

Rabbit Total Endothelin ELISA kit

Species

Rabbit

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ET ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- ET antibody and an ET-HRP conjugate. The assay sample and buffer are incubated together with ET-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ET concentration since ET from samples and ET-HRP conjugate compete for the anti-ET antibody binding site. Since the number of sites is limited, as more sites are occupied by ET from the sample, fewer sites are left to bind ET-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ET concentration in each sample is interpolated from this standard curve.


Quality Control on Rabbit Total Endothelin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-114.79%

Linearity

Diluent Ratio

Range %

1:2

96-116

1:4

91-109

1:8

88-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ET and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit Total Endothelin ELISA kit

Summary of the Assay Procedure for Rabbit Total Endothelin ELISA kit

Citations of Rabbit Total Endothelin ELISA kit

E04E0182 has been referenced in the below publications:

The effect of nitriglycerin on the microcirculation disorder in the acute pancreatitis of rabbits.

Effect of extracorporeal shock wave on endothelin-1 expression and content in blood of glucocorticoid-induced femoral head necrosis in rabbits.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.