E04E0032 Rabbit Total Epidermal Growth Factor ELISA kit
Rabbit Total Epidermal Growth Factor ELISA kit is suitable for detecting samples from rabbit species. Epidermal Growth Factor can also be called EGF; Beta urogastrone; Epidermal Growth Factor; Pro epidermal growth factor; URG; Urogastrone; HOMG4.
Product Information | |
Cat. No. | E04E0032 |
Product Name | Rabbit Total Epidermal Growth Factor ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
EGF ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- EGF antibody and an EGF-HRP conjugate. The assay sample and buffer are incubated together with EGF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EGF concentration since EGF from samples and EGF-HRP conjugate compete for the anti-EGF antibody binding site. Since the number of sites is limited, as more sites are occupied by EGF from the sample, fewer sites are left to bind EGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EGF concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-114.79% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-116 | |
1:4 | 91-109 | |
1:8 | 88-103 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between EGF and analogues was observed. | |
E04E0032 has been referenced in the below publications:
Effect of Banxia Houpu Decoction on EGF and GasConcentration in Peripheral Blood of Rabbits with Vomit Caused By Chemotherapy.
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