En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat 25 hydroxyvitamin D3 ELISA Kit (E02O0874)

E02O0874 Rat 25 hydroxyvitamin D3 ELISA Kit

The Rat 25 hydroxyvitamin D3 ELISA Kit can be used to identify samples from the rat species. 25 hydroxyvitamin D3 can also be called 25-Hydroxyvitamin D3, 25-Hydroxycholecalciferol, Calcidiol, 25(OH)D3.

Products

Specifications of Rat 25 hydroxyvitamin D3 ELISA Kit

Product Information

Cat. No.

E02O0874

Product Name

Rat 25 hydroxyvitamin D3 ELISA Kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

10-250 ng/mL

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

10 ng/mL

1 vial

STANDARD C (0.5mL)

25 ng/mL

1 vial

STANDARD D (0.5mL)

50 ng/mL

1 vial

STANDARD E (0.5mL)

100 ng/mL

1 vial

STANDARD F (0.5mL)

250 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

25(OH)D3 ELISA kit uses an anti-25(OH)D3 antibody and an 25(OH)D3-HRP conjugate in a competitive enzyme immunoassay method. 25(OH)D3-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-25(OH)D3 antibody binding site between 25(OH)D3 from samples and 25(OH)D3-HRP conjugate, the intensity of the color is inversely proportional to the concentration of 25(OH)D3. Since the number of sites is limited, as more sites are occupied by 25(OH)D3 from the sample, fewer sites are left to bind 25(OH)D3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 25(OH)D3 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat 25 hydroxyvitamin D3 ELISA Kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between 25(OH)D3 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat 25 hydroxyvitamin D3 ELISA Kit

Summary of the Assay Procedure for Rat 25 hydroxyvitamin D3 ELISA Kit

Citations of Rat 25 hydroxyvitamin D3 ELISA Kit

E02O0874 has been referenced in the below publications:

Cardiac metabolism, inflammation, and peroxisome proliferator-activated receptors modulated by 1, 25-dihydroxyvitamin D3 in diabetic rats.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.