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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat 3 Nitrotyrosine ELISA kit (E02A0670)

E02A0670 Rat 3 Nitrotyrosine ELISA kit

The Rat 3 Nitrotyrosine ELISA kit can be used to identify samples from the rat species. 3 Nitrotyrosine can also be called 3-Nitro-L-tyrosine, 3-Nitrotyrosine, 3 NT.

Products

Specifications of Rat 3 Nitrotyrosine ELISA kit

Product Information

Cat. No.

E02A0670

Product Name

Rat 3 Nitrotyrosine ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

3 NT ELISA kit uses an anti-3 NT antibody and an 3 NT-HRP conjugate in a competitive enzyme immunoassay method. 3 NT-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-3 NT antibody binding site between 3 NT from samples and 3 NT-HRP conjugate, the intensity of the color is inversely proportional to the concentration of 3 NT. Since the number of sites is limited, as more sites are occupied by 3 NT from the sample, fewer sites are left to bind 3 NT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 3 NT concentration in each sample is interpolated from this standard curve.


Quality Control on Rat 3 Nitrotyrosine ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between 3 NT and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat 3 Nitrotyrosine ELISA kit

Summary of the Assay Procedure for Rat 3 Nitrotyrosine ELISA kit

Citations of Rat 3 Nitrotyrosine ELISA kit

E02A0670 has been referenced in the below publications:

Carnosine Attenuates Brain Oxidative Stress and Apoptosis After Intracerebral Hemorrhage in Rats.

Effect of Gastrodin on Early Brain Injury and Neurological Outcome After Subarachnoid Hemorrhage in Rats.

Tempol ameliorates polycystic ovary syndrome through attenuating intestinal oxidative stress and modulating of gut microbiota composition-serum metabolites interaction.

Treatment with 3-aminobenzamide during ex vivo lung perfusion of damaged rat lungs reduces graft injury and dysfunction after transplantation.

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