E02A0670 Rat 3 Nitrotyrosine ELISA kit
The Rat 3 Nitrotyrosine ELISA kit can be used to identify samples from the rat species. 3 Nitrotyrosine can also be called 3-Nitro-L-tyrosine, 3-Nitrotyrosine, 3 NT.
Specifications of Rat 3 Nitrotyrosine ELISA kit
Product Information | |
Cat. No. | E02A0670 |
Product Name | Rat 3 Nitrotyrosine ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
3 NT ELISA kit uses an anti-3 NT antibody and an 3 NT-HRP conjugate in a competitive enzyme immunoassay method. 3 NT-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-3 NT antibody binding site between 3 NT from samples and 3 NT-HRP conjugate, the intensity of the color is inversely proportional to the concentration of 3 NT. Since the number of sites is limited, as more sites are occupied by 3 NT from the sample, fewer sites are left to bind 3 NT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 3 NT concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat 3 Nitrotyrosine ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between 3 NT and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat 3 Nitrotyrosine ELISA kit
Citations of Rat 3 Nitrotyrosine ELISA kit
E02A0670 has been referenced in the below publications：
Carnosine Attenuates Brain Oxidative Stress and Apoptosis After Intracerebral Hemorrhage in Rats.
Effect of Gastrodin on Early Brain Injury and Neurological Outcome After Subarachnoid Hemorrhage in Rats.
Tempol ameliorates polycystic ovary syndrome through attenuating intestinal oxidative stress and modulating of gut microbiota composition-serum metabolites interaction.
Treatment with 3-aminobenzamide during ex vivo lung perfusion of damaged rat lungs reduces graft injury and dysfunction after transplantation.
