E02H0203 Rat 4 Hydroxynonenal ELISA kit
The Rat 4 Hydroxynonenal ELISA kit can be used to identify samples from the rat species. 4 Hydroxynonenal can also be called 4-Hydroxy-2-Nonenal, 4Hydroxynonenal, 4-Hydroxynonenal, (E)-4-Hydroxynonenal-dimethylacetal, 4-HNE-DMA, 4 HNE.
Product Information | |
Cat. No. | E02H0203 |
Product Name | Rat 4 Hydroxynonenal ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ug/ml |
Sensitivity | 0.1 ug/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ug/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ug/mL | 1 vial |
STANDARD D (0.5mL) | 10 ug/mL | 1 vial |
STANDARD E (0.5mL) | 25 ug/mL | 1 vial |
STANDARD F (0.5mL) | 50 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
4 HNE ELISA kit uses an anti-I4 HNE antibody and an I4 HNE-HRP conjugate in a competitive enzyme immunoassay method. I4 HNE-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-I4 HNE antibody binding site between I4 HNE from samples and I4 HNE-HRP conjugate, the intensity of the color is inversely proportional to the concentration of I4 HNE. Since the number of sites is limited, as more sites are occupied by I4 HNE from the sample, fewer sites are left to bind I4 HNE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The I4 HNE concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between 4 HNE and analogues was observed. | |
E02H0203 has been referenced in the below publications：
Beneficial effects of intravenous iron therapy in a rat model of heart failure with preserved systemic iron status but depleted intracellular cardiac stores.
Effects of Adiponectin on Emphysema of Rats Induced by Different Ways of Rearing and Tobacco Smoke.
The role of oxidative stress caused by different feeding manners in the formation of emphysema in rats.
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