E02H0320 Rat 5 Hydroxyindoleacetic Acid ELISA kit
The Rat 5 Hydroxyindoleacetic Acid ELISA kit can be used to identify samples from the rat species. 5 Hydroxyindoleacetic Acid can also be called (5-Hydroxy-1H-indol-3-yl)acetic acid, 5 HIAA.
Specifications of Rat 5 Hydroxyindoleacetic Acid ELISA kit
Product Information | |
Cat. No. | E02H0320 |
Product Name | Rat 5 Hydroxyindoleacetic Acid ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 50 ng/mL | 1 vial |
STANDARD C (0.5mL) | 100 ng/mL | 1 vial |
STANDARD D (0.5mL) | 250 ng/mL | 1 vial |
STANDARD E (0.5mL) | 500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
5 HIAA ELISA kit uses an anti-5 HIAA antibody and an 5 HIAA-HRP conjugate in a competitive enzyme immunoassay method. 5 HIAA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-5 HIAA antibody binding site between 5 HIAA from samples and 5 HIAA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of 5 HIAA. Since the number of sites is limited, as more sites are occupied by 5 HIAA from the sample, fewer sites are left to bind 5 HIAA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 5 HIAA concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat 5 Hydroxyindoleacetic Acid ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between 5 HIAA and analogues was observed. | |
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Summary of the Assay Procedure for Rat 5 Hydroxyindoleacetic Acid ELISA kit
Citations of Rat 5 Hydroxyindoleacetic Acid ELISA kit
E02H0320 has been referenced in the below publications：
参麦黄连阿胶汤治疗绝经综合征的药效学实验。
Multi-omic Profiling Reveals that Intra-abdominal Hypertension-Induced Intestinal Damage Can Be Prevented by Microbiome and Metabolic Modulations with 5-Hydroxyindoleacetic Acid as a Diagnostic Marker.
