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  • bovine serum albumin elisa 1

BlueGene Biotech Rat 5 Hydroxytryptamine ELISA kit (E02H0106)

E02H0106 Rat Serotonin/5 Hydroxytryptamine ELISA kit

The Rat Serotonin/5 Hydroxytryptamine ELISA kit can be used to identify samples from the rat species. Serotonin/5 Hydroxytryptamine  can also be called 5HT, Serotonin, N-Methyl-Gamma, 5 HT, 5HT.

Products

Specifications of Rat Serotonin/5 Hydroxytryptamine ELISA kit

Product Information

Cat. No.

E02H0106

Product Name

Rat Serotonin/5 Hydroxytryptamine ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

250-5000 ng/ml

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

250 ng/mL

1 vial

STANDARD C (0.5mL)

500 ng/mL

1 vial

STANDARD D (0.5mL)

1000 ng/mL

1 vial

STANDARD E (0.5mL)

2500 ng/mL

1 vial

STANDARD F (0.5mL)

5000 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

5 HT ELISA kit uses an anti-I5 HT antibody and an I5 HT-HRP conjugate in a competitive enzyme immunoassay method. I5 HT-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-I5 HT antibody binding site between I5 HT from samples and I5 HT-HRP conjugate, the intensity of the color is inversely proportional to the concentration of I5 HT. Since the number of sites is limited, as more sites are occupied by I5 HT from the sample, fewer sites are left to bind I5 HT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The I5 HT concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Serotonin/5 Hydroxytryptamine ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between 5 HT and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Serotonin/5 Hydroxytryptamine ELISA kit

Summary of the Assay Procedure for Rat Serotonin/5 Hydroxytryptamine ELISA kit

Citations of Rat Serotonin/5 Hydroxytryptamine ELISA kit

E02H0106 has been referenced in the below publications:

Effect of Self-made Changpu Yujin Decoction on Monoamine Neurotransmitters and Dopamine System in TS Model Rats.

EXPERIMENTAL STUDY ON BUSHENNING REGULATING RATNEUROTRANSMITTERS AND REPRODUCTIVE FUNCTION IN THE OVULATION INHIBITION OF KIDNEY YANG.

Observation of Electroacupuncture at Zusanli (St36) and Quchi (Li 11) on Two Peaks Blood Pressure and Serum Melatonin and Serotonin in Spontaneously Hypertensive Rats.

Effects of Wenshen Jianpi Decoction on 5-hydroxytryptamine and its receptors in colon mucosa of rats with diarrhea-predominant irritable bowel syndrome.

藜欢解郁胶囊的抗抑郁作用。

参麦黄连阿胶汤治疗绝经综合征的药效学实验。

SiRNA-mediated serotonin transporter knockdown in the dorsal raphe nucleus rescues single prolonged stress-induced hippocampal autophagy in rats.

Multi-omic Profiling Reveals that Intra-abdominal Hypertension-Induced Intestinal Damage Can Be Prevented by Microbiome and Metabolic Modulations with 5-Hydroxyindoleacetic Acid as a Diagnostic Marker.


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