E02H0106 Rat Serotonin/5 Hydroxytryptamine ELISA kit
The Rat Serotonin/5 Hydroxytryptamine ELISA kit can be used to identify samples from the rat species. Serotonin/5 Hydroxytryptamine can also be called 5HT, Serotonin, N-Methyl-Gamma, 5 HT, 5HT.
Specifications of Rat Serotonin/5 Hydroxytryptamine ELISA kit
Product Information | |
Cat. No. | E02H0106 |
Product Name | Rat Serotonin/5 Hydroxytryptamine ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000 ng/ml |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 250 ng/mL | 1 vial |
STANDARD C (0.5mL) | 500 ng/mL | 1 vial |
STANDARD D (0.5mL) | 1000 ng/mL | 1 vial |
STANDARD E (0.5mL) | 2500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 5000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
5 HT ELISA kit uses an anti-I5 HT antibody and an I5 HT-HRP conjugate in a competitive enzyme immunoassay method. I5 HT-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-I5 HT antibody binding site between I5 HT from samples and I5 HT-HRP conjugate, the intensity of the color is inversely proportional to the concentration of I5 HT. Since the number of sites is limited, as more sites are occupied by I5 HT from the sample, fewer sites are left to bind I5 HT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The I5 HT concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Serotonin/5 Hydroxytryptamine ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between 5 HT and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Serotonin/5 Hydroxytryptamine ELISA kit
Citations of Rat Serotonin/5 Hydroxytryptamine ELISA kit
E02H0106 has been referenced in the below publications：
Effect of Self-made Changpu Yujin Decoction on Monoamine Neurotransmitters and Dopamine System in TS Model Rats.
EXPERIMENTAL STUDY ON BUSHENNING REGULATING RATNEUROTRANSMITTERS AND REPRODUCTIVE FUNCTION IN THE OVULATION INHIBITION OF KIDNEY YANG.
Observation of Electroacupuncture at Zusanli (St36) and Quchi (Li 11) on Two Peaks Blood Pressure and Serum Melatonin and Serotonin in Spontaneously Hypertensive Rats.
Effects of Wenshen Jianpi Decoction on 5-hydroxytryptamine and its receptors in colon mucosa of rats with diarrhea-predominant irritable bowel syndrome.
藜欢解郁胶囊的抗抑郁作用。
参麦黄连阿胶汤治疗绝经综合征的药效学实验。
SiRNA-mediated serotonin transporter knockdown in the dorsal raphe nucleus rescues single prolonged stress-induced hippocampal autophagy in rats.
Multi-omic Profiling Reveals that Intra-abdominal Hypertension-Induced Intestinal Damage Can Be Prevented by Microbiome and Metabolic Modulations with 5-Hydroxyindoleacetic Acid as a Diagnostic Marker.
