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BlueGene Biotech Rat 8 Hydroxy 2 desoxyguanosine ELISA kit (E02H0007)

E02H0007 Rat 8 Hydroxy 2 desoxyguanosine ELISA kit

The Rat 8 Hydroxy 2 desoxyguanosine ELISA kit can be used to identify samples from the rat species. 8 Hydroxy 2 desoxyguanosine can also be called 8 Hydroxyguanosine, 8-Hydroxy-2'-deoxyguanosine, 8-Hydroxydeoxyguanosine, 8 hydroxy 2’ deoxyguanosine, 8 hydroxyguanine, 8 hydroxyguanosine, 8 OHG, 8-OHG, 8OG, 8OHdG, 8OHG, 8 Oxoguanine, 8 hydroxyguanine.

Products

Specifications of Rat 8 Hydroxy 2 desoxyguanosine ELISA kit

Product Information

Cat. No.

E02H0007

Product Name

Rat 8 Hydroxy 2 desoxyguanosine ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

8 OHdG ELISA kit uses an anti-8 OHdG antibody and an 8 OHdG-HRP conjugate in a competitive enzyme immunoassay method. 8 OHdG-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-8 OHdG antibody binding site between 8 OHdG from samples and 8 OHdG-HRP conjugate, the intensity of the color is inversely proportional to the concentration of 8 OHdG. Since the number of sites is limited, as more sites are occupied by 8 OHdG from the sample, fewer sites are left to bind 8 OHdG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 8 OHdG concentration in each sample is interpolated from this standard curve.


Quality Control on Rat 8 Hydroxy 2 desoxyguanosine ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between 8 OHdG and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat 8 Hydroxy 2 desoxyguanosine ELISA kit

Summary of the Assay Procedure for Rat 8 Hydroxy 2 desoxyguanosine ELISA kit

Citations of Rat 8 Hydroxy 2 desoxyguanosine ELISA kit

E02H0007 has been referenced in the below publications:

Protective effects of hydrogen-rich saline on monocrotaline-induced pulmonary hypertension in a rat model.

Spatial Distribution of 5-Hydroxymethyl Cytosine in Rat Brain and Temporal Distribution in Striatum.

Hydroxysafflor yellow A improves established monocrotaline-induced pulmonary arterial hypertension in rats.

Identification of potential biomarkers of gold nanoparticle toxicity in rat brains.

Oral exposure to dibutyl phthalate exacerbates chronic lymphocytic thyroiditis through oxidative stress in female Wistar rats.

Influence of Heart-Protection Musk Pill on Inflammatory Factor in Myocardial Tissue in Rats with Acute Myocardial Infarction.

THE PROTECTIVE EFFECT OF HYDROGEN-SATURATED SALINE ONCARDIOVASCULAR HYPERTROPHY IN SINOAORTIC DENERVATION RATS.

THE PROTECTIVE EFEECT OF HYDROGEN-RICH SALINE ON ISOPROTERENOL-INDUCEDACUTE MYOCARDIAL INFARCTION IN RAT.

Fluoxetine is Neuroprotective in Early Brain Injury via its Anti-inflammatory and Anti-apoptotic Effects in a Rat Experimental Subarachnoid Hemorrhage Model.

Carnosine Attenuates Brain Oxidative Stress and Apoptosis After Intracerebral Hemorrhage in Rats.


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