Rat Acetyl Coenzyme A Acetyltransferase 2 ELISA kit (E02A0648)

BlueGene Biotech Rat Acetyl Coenzyme A Acetyltransferase 2 ELISA kit (E02A0648)

E02A0648 Rat Acetyl Coenzyme A Acetyltransferase 2 ELISA kit

The Rat Acetyl Coenzyme A Acetyltransferase 2 ELISA kit can be used to identify samples from the rat species. Acetyl Coenzyme A Acetyltransferase 2 can also be called ACAT2, acetyl-CoA acetyltransferase 2;cytosolic acetoacetyl-CoA thiolase.

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Specifications of Rat Acetyl Coenzyme A Acetyltransferase 2 ELISA kit

Product Information
Cat. No.	E02A0648
Product Name	Rat Acetyl Coenzyme A Acetyltransferase 2 ELISA kit
Species	Rat
Product Size	48 Tests / 96 Tests
Concentration	50-1000 pg/ml
Sensitivity	1.0 pg/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/mL	1 vial
STANDARD B (0.5mL)	50 pg/mL	1 vial
STANDARD C (0.5mL)	100 pg/mL	1 vial
STANDARD D (0.5mL)	250 pg/mL	1 vial
STANDARD E (0.5mL)	500 pg/mL	1 vial
STANDARD F (0.5mL)	1000 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

ACAT2 ELISA kit uses an anti-ACAT2 antibody and an ACAT2-HRP conjugate in a competitive enzyme immunoassay method. ACAT2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ACAT2 antibody binding site between ACAT2 from samples and ACAT2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ACAT2. Since the number of sites is limited, as more sites are occupied by ACAT2 from the sample, fewer sites are left to bind ACAT2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACAT2 concentration in each sample is interpolated from this standard curve.

Quality Control on Rat Acetyl Coenzyme A Acetyltransferase 2 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between ACAT2 and analogues was observed.