E02A0632 Rat Adenosine Deaminase ELISA kit
The Rat Adenosine Deaminase ELISA kit can be used to identify samples from the rat species. Adenosine Deaminase can also be called ADA, entrez:100, Adenosine deaminase, ADA1.
Specifications of Rat Adenosine Deaminase ELISA kit
Product Information | |
Cat. No. | E02A0632 |
Product Name | Rat Adenosine Deaminase ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/mL |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ADA ELISA kit uses an anti-ADA antibody and an ADA-HRP conjugate in a competitive enzyme immunoassay method. ADA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ADA antibody binding site between ADA from samples and ADA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ADA. Since the number of sites is limited, as more sites are occupied by ADA from the sample, fewer sites are left to bind ADA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADA concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Adenosine Deaminase ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ADA and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Adenosine Deaminase ELISA kit
Citations of Rat Adenosine Deaminase ELISA kit
E02A0632 has been referenced in the below publications：
Systemic Wound Healing Associated with local sub-Cutaneous Mechanical Stimulation.
