E02A0388 Rat Alanine Aminotransferase ELISA kit
The Rat Alanine Aminotransferase ELISA kit can be used to identify samples from the rat species. Alanine Aminotransferase can also be called Alanine aminotransferase, AAT 1, AAT1, Alanine aminotransferase 1, alanine aminotransferase, ALT 1, ALT1, Glutamic alanine transaminase 1, Glutamic pyruvate transaminase, Glutamic pyruvic transaminase 1, Glutamic-alanine transaminase, GPT 1, GPT, GPT1, SGPT, ALAT1.
Product Information | |
Cat. No. | E02A0388 |
Product Name | Rat Alanine Aminotransferase ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ALT/GPT ELISA kit uses an anti-ALT/GPT antibody and an ALT/GPT-HRP conjugate in a competitive enzyme immunoassay method. ALT/GPT-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ALT/GPT antibody binding site between ALT/GPT from samples and ALT/GPT-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ALT/GPT. Since the number of sites is limited, as more sites are occupied by ALT/GPT from the sample, fewer sites are left to bind ALT/GPT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALT/GPT concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ALT/GPT and analogues was observed. | |
E02A0388 has been referenced in the below publications：
Oral administration of oleanolic acid, isolated from Swertia mussotii Franch, attenuates liver injury,inflammation, and cholestasis in bile duct-ligated rats.
Swertianlarin, isolated from Swertia mussotii Franch, increases detoxification enzymes and efflux transporters expression in rats.
Protective effects and mechanisms of betaine on liver cirrhosis induced by multiple pathogenic factors in rats.
Effects of macrophage polarization during development of liver cirrhosis in rats.
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