E02A0774 Rat Aldosterone ELISA kit
The Rat Aldosterone ELISA kit can be used to identify samples from the rat species. Aldosterone can also be called Aldocorten, Aldocortin, Electrocortin, Reichstein X, 18-Aldocorticosterone, 18-Oxocorticosterone, 11β,21-Dihydroxy-3,20-dioxopregn-4-en-18-al, ALD.
Product Information | |
Cat. No. | E02A0774 |
Product Name | Rat Aldosterone ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500 pg/mL |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ALD ELISA kit uses an anti-ALD antibody and an ALD-HRP conjugate in a competitive enzyme immunoassay method. ALD-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ALD antibody binding site between ALD from samples and ALD-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ALD. Since the number of sites is limited, as more sites are occupied by ALD from the sample, fewer sites are left to bind ALD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALD concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ALD and analogues was observed. | |
E02A0774 has been referenced in the below publications：
The effect of different dosing schedule of perindopril on circadian rhythm of urine rennin-angiotensin system in spontaneously hypertensive rats.
The influence of recombinant human brain natriuretic peptide and furosemide on diuretic effect in rats with heart failure with reduced ejection fraction and its mechanism.
Phosphorylation of CaMK and CREB-Mediated Cardiac Aldosterone Synthesis Induced by Arginine Vasopressin in Rats with Myocardial Infarction.
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