E02S0004 Rat Alpha Smooth Muscle Actin ELISA kit
The Rat Alpha Smooth Muscle Actin ELISA kit can be used to identify samples from the rat species. Alpha Smooth Muscle Actin can also be called AAT6, ACTSA, MYMY5, Alpha SMA.
Specifications of Rat Alpha Smooth Muscle Actin ELISA kit
Product Information | |
Cat. No. | E02S0004 |
Product Name | Rat Alpha Smooth Muscle Actin ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 ng/mL |
Sensitivity | 1.0 ng/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 50 ng/ml | 1 vial |
STANDARD C (0.5mL) | 100 ng/ml | 1 vial |
STANDARD D (0.5mL) | 250 ng/ml | 1 vial |
STANDARD E (0.5mL) | 500 ng/ml | 1 vial |
STANDARD F (0.5mL) | 1000 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Alpha SMA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Alpha SMA. Standards or samples are then added to the microtiter plate wells and Alpha SMA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of Alpha SMA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for Alpha SMA are added to each well to “sandwich” the Alpha SMA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Alpha SMA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Alpha SMA concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Alpha Smooth Muscle Actin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Alpha SMA and analogues was observed. | |
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Summary of the Assay Procedure for Rat Alpha Smooth Muscle Actin ELISA kit
Citations of Rat Alpha Smooth Muscle Actin ELISA kit
E02S0004 has been referenced in the below publications：
Discussion of changes of TGF-β 1，Smad-4，α-SMA and PDGF in DN rat liver before and after treatment in traditional Chinese Medicine based on the“liver and kidney share the same origin”.
