E02A0049 Rat Amyloid β Protein 40 ELISA kit
The Rat Amyloid β Protein 40 ELISA kit can be used to identify samples from the rat species. Amyloid β Protein 40 can also be called Beta-APP40;Beta-amyloid protein 40, ABPP, APPI, Amyloid beta A4 protein, MOAB2, MOAB-2, Alzheimer's antibody, AB40, Aβ40.
Product Information | |
Cat. No. | E02A0049 |
Product Name | Rat Amyloid β Protein 40 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Aβ40 ELISA kit uses an anti-Aβ40 antibody and an Aβ40-HRP conjugate in a competitive enzyme immunoassay method. Aβ40-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Aβ40 antibody binding site between Aβ40 from samples and Aβ40-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Aβ40. Since the number of sites is limited, as more sites are occupied by Aβ40 from the sample, fewer sites are left to bind Aβ40-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Aβ40 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Aβ40 and analogues was observed. | |
E02A0049 has been referenced in the below publications：
Roliram improved cognitive impairment and neuroinflammation in diabetic encephalopathy.
Protective effects of BDNF on hippocampal neurons under high glucose condition.
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