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  • bovine serum albumin elisa 1

BlueGene Biotech Rat Amyloid β Protein 40 ELISA kit (E02A0049)

E02A0049 Rat Amyloid β Protein 40 ELISA kit

The Rat Amyloid β Protein 40 ELISA kit can be used to identify samples from the rat species. Amyloid β Protein 40 can also be called Beta-APP40;Beta-amyloid protein 40, ABPP, APPI, Amyloid beta A4 protein, MOAB2, MOAB-2, Alzheimer's antibody, AB40, Aβ40.

Products

Specifications of Rat Amyloid β Protein 40 ELISA kit

Product Information

Cat. No.

E02A0049

Product Name

Rat Amyloid β Protein 40 ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Aβ40 ELISA kit uses an anti-Aβ40 antibody and an Aβ40-HRP conjugate in a competitive enzyme immunoassay method. Aβ40-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Aβ40 antibody binding site between Aβ40 from samples and Aβ40-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Aβ40. Since the number of sites is limited, as more sites are occupied by Aβ40 from the sample, fewer sites are left to bind Aβ40-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Aβ40 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Amyloid β Protein 40 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Aβ40 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Amyloid β Protein 40 ELISA kit

Summary of the Assay Procedure for Rat Amyloid β Protein 40 ELISA kit

Citations of Rat Amyloid β Protein 40 ELISA kit

E02A0049 has been referenced in the below publications:

Roliram improved cognitive impairment and neuroinflammation in diabetic encephalopathy.

Protective effects of BDNF on hippocampal neurons under high glucose condition.


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