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BlueGene Biotech Rat Amyloid β Protein 42 ELISA kit (E02A0050)

E02A0050 Rat Amyloid β Protein 42 ELISA kit

The Rat Amyloid β Protein 42 ELISA kit can be used to identify samples from the rat species. Amyloid β Protein 42 can also be called beta Amyloid(1-42), beta Amyloid 1-42, beta-Amyloid 1-42, Amyloid 1-42, beta-Amyloid 42, A4, AAA, ABETA, ABPP, AD1, Alzheimers Disease Amyloid Protein, Amyloid B, Amyloid Beta A4 Protein Precursor, Aβ42.

Products

Specifications of Rat Amyloid β Protein 42 ELISA kit

Product Information

Cat. No.

E02A0050

Product Name

Rat Amyloid β Protein 42 ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 pg/ml

1 vial

STANDARD B (0.5mL)

50 pg/ml

1 vial

STANDARD C (0.5mL)

100 pg/ml

1 vial

STANDARD D (0.5mL)

250 pg/ml

1 vial

STANDARD E (0.5mL)

500 pg/ml

1 vial

STANDARD F (0.5mL)

1000 pg/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Aβ42 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Aβ42. Standards or samples are then added to the microtiter plate wells and Aβ42 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of Aβ42 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for Aβ42 are added to each well to “sandwich” the Aβ42 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Aβ42 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Aβ42 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Amyloid β Protein 42 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Aβ42 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Amyloid β Protein 42 ELISA kit

Summary of the Assay Procedure for Rat Amyloid β Protein 42 ELISA kit

Citations of Rat Amyloid β Protein 42 ELISA kit

E02A0050 has been referenced in the below publications:

Alteration in amyloid β42, phosphorylated tau protein, interleukin 6, and acetylcholine during diabetes-accelerated memory dysfunction in diabetic rats: correlation of amyloid β42 with changes in glucose metabolism.

Roliram improved cognitive impairment and neuroinflammation in diabetic encephalopathy.

Protective effects of BDNF on hippocampal neurons under high glucose condition.

Roliram improved cognitive impairment and neuroinflammation in diabetic encephalopathy.

A study on correlation of cognitive function and expression with beta-amyloid,tumor necrosis factor alpha in hippocampus of diabetic rats.

Dynamic changes of major biomarkers in rats plasma during the development of diabete.

Expression of Arc/Arg3.1 in diabetic rat hippocampus and its relationship with cognitive function.

Alteration in amyloid β42,phosphorylated tau protein, interleukin 6,and acetylcholine during diabetes-accelerated memory dysfunction in diabetic rats: correlation of amyloid β42 with changes in glucose metabolismb.


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