Rat Amyloid β Protein 42 ELISA kit (E02A0050) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Rat Amyloid β Protein 42 ELISA kit (E02A0050)

E02A0050 Rat Amyloid β Protein 42 ELISA kit

The Rat Amyloid β Protein 42 ELISA kit can be used to identify samples from the rat species. Amyloid β Protein 42 can also be called beta Amyloid(1-42), beta Amyloid 1-42, beta-Amyloid 1-42, Amyloid 1-42, beta-Amyloid 42, A4, AAA, ABETA, ABPP, AD1, Alzheimers Disease Amyloid Protein, Amyloid B, Amyloid Beta A4 Protein Precursor, Aβ42.

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Specifications of Rat Amyloid β Protein 42 ELISA kit

Product Information
Cat. No.	E02A0050
Product Name	Rat Amyloid β Protein 42 ELISA kit
Species	Rat
Product Size	48 Tests / 96 Tests
Concentration	50-1000 pg/mL
Sensitivity	1.0 pg/ml
Principal	Sandwich ELISA
Sample Volume	50 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	10 mL	1 vial
STANDARD A (0.5mL)	0 pg/ml	1 vial
STANDARD B (0.5mL)	50 pg/ml	1 vial
STANDARD C (0.5mL)	100 pg/ml	1 vial
STANDARD D (0.5mL)	250 pg/ml	1 vial
STANDARD E (0.5mL)	500 pg/ml	1 vial
STANDARD F (0.5mL)	1000 pg/ml	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

Aβ42 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Aβ42. Standards or samples are then added to the microtiter plate wells and Aβ42 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of Aβ42 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for Aβ42 are added to each well to “sandwich” the Aβ42 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Aβ42 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Aβ42 concentration in each sample is interpolated from this standard curve.

Quality Control on Rat Amyloid β Protein 42 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between Aβ42 and analogues was observed.

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Summary of the Assay Procedure for Rat Amyloid β Protein 42 ELISA kit

Citations of Rat Amyloid β Protein 42 ELISA kit

E02A0050 has been referenced in the below publications：

Alteration in amyloid β42, phosphorylated tau protein, interleukin 6, and acetylcholine during diabetes-accelerated memory dysfunction in diabetic rats: correlation of amyloid β42 with changes in glucose metabolism.

Roliram improved cognitive impairment and neuroinflammation in diabetic encephalopathy.

Protective effects of BDNF on hippocampal neurons under high glucose condition.

Roliram improved cognitive impairment and neuroinflammation in diabetic encephalopathy.

A study on correlation of cognitive function and expression with beta-amyloid，tumor necrosis factor alpha in hippocampus of diabetic rats.

Dynamic changes of major biomarkers in rats plasma during the development of diabete.

Expression of Arc/Arg3.1 in diabetic rat hippocampus and its relationship with cognitive function.

Alteration in amyloid β42,phosphorylated tau protein, interleukin 6,and acetylcholine during diabetes-accelerated memory dysfunction in diabetic rats: correlation of amyloid β42 with changes in glucose metabolismb.