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  • bovine serum albumin elisa 1

BlueGene Biotech Rat amyloid beta peptide 25-35 ELISA Kit (E02A1781)

E02A1781 Rat amyloid beta peptide 25-35 ELISA Kit

The Rat amyloid beta peptide 25-35 ELISA Kit can be used to identify samples from the rat species. amyloid beta peptide 25-35 can also be called Abeta25-35, Ab 25-35, Abeta 25-35

Products

Specifications of Rat amyloid beta peptide 25-35 ELISA Kit

Product Information

Cat. No.

E02A1781

Product Name

Rat amyloid beta peptide 25-35 ELISA Kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

100-2500 pg/mL

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

100 pg/mL

1 vial

STANDARD C (0.5mL)

250 pg/mL

1 vial

STANDARD D (0.5mL)

500 pg/mL

1 vial

STANDARD E (0.5mL)

1000 pg/mL

1 vial

STANDARD F (0.5mL)

2500 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Aβ25-35 ELISA kit uses an anti-Aβ25-35 antibody and an Aβ25-35-HRP conjugate in a competitive enzyme immunoassay method. Aβ25-35-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Aβ25-35 antibody binding site between Aβ25-35 from samples and Aβ25-35-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Aβ25-35. Since the number of sites is limited, as more sites are occupied by Aβ25-35 from the sample, fewer sites are left to bind Aβ25-35-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Aβ25-35 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat amyloid beta peptide 25-35 ELISA Kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Aβ25-35 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat amyloid beta peptide 25-35 ELISA Kit

Summary of the Assay Procedure for Rat amyloid beta peptide 25-35 ELISA Kit

Citations of Rat amyloid beta peptide 25-35 ELISA Kit

E02A1781 has been referenced in the below publications:

Reduction in AB-induced cell death in thehippocampus of 17B-estradiol-treated female ratsis associated with an increase in IGF-I signalingand somatostatinergic tone.


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