Rat Angiopoietin Like Protein 4 ELISA kit (E02A0507) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Rat Angiopoietin Like Protein 4 ELISA kit (E02A0507)

E02A0507 Rat Angiopoietin Like Protein 4 ELISA kit

The Rat Angiopoietin Like Protein 4 ELISA kit can be used to identify samples from the rat species. Angiopoietin Like Protein 4 can also be called ANGPTL4, ANGPTL2, ARP4, FIAF, HARP, HFARP, NL2, PGAR, TGQTL, UNQ171, pp1158, angiopoietin like 4

Products

Specifications of Rat Angiopoietin Like Protein 4 ELISA kit

Product Information
Cat. No.	E02A0507
Product Name	Rat Angiopoietin Like Protein 4 ELISA kit
Species	Rat
Product Size	48 Tests / 96 Tests
Concentration	0.5-10 ng/mL
Sensitivity	0.1 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	0.5 ng/mL	1 vial
STANDARD C (0.5mL)	1.0 ng/mL	1 vial
STANDARD D (0.5mL)	2.5 ng/mL	1 vial
STANDARD E (0.5mL)	5.0 ng/mL	1 vial
STANDARD F (0.5mL)	10 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

ANGPTL4 ELISA kit uses an anti-ANGPTL4 antibody and an ANGPTL4-HRP conjugate in a competitive enzyme immunoassay method. ANGPTL4-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ANGPTL4 antibody binding site between ANGPTL4 from samples and ANGPTL4-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ANGPTL4. Since the number of sites is limited, as more sites are occupied by ANGPTL4 from the sample, fewer sites are left to bind ANGPTL4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANGPTL4 concentration in each sample is interpolated from this standard curve.

Quality Control on Rat Angiopoietin Like Protein 4 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between ANGPTL4 and analogues was observed.

BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Angiopoietin Like Protein 4 ELISA kit

Citations of Rat Angiopoietin Like Protein 4 ELISA kit

E02A0507 has been referenced in the below publications：

Effect of Jianpi Yiqi Huoxue Decoction on SHR Releasing Vasoactive Substances by Regulating ACE2-Ang-(l-7)-MAS-AKT Signal Pathways Regulate Releasing Vasoactive Substances.