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BlueGene Biotech Rat Angiotensin (1-7) ELISA kit (E02A0206)

E02A0206 Rat Angiotensin (1-7) ELISA kit

The Rat Angiotensin (1-7) ELISA kit can be used to identify samples from the rat species. Angiotensin (1-7)  can also be called Angiotensin I (1-7), Ang-(1-7), Ang1-7, ANG (1 7).

Products

Specifications of Rat Angiotensin (1-7) ELISA kit

Product Information

Cat. No.

E02A0206

Product Name

Rat Angiotensin (1-7) ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ANG (1 7) ELISA kit uses an anti-ANG (1 7) antibody and an ANG (1 7)-HRP conjugate in a competitive enzyme immunoassay method. ANG (1 7)-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ANG (1 7) antibody binding site between ANG (1 7) from samples and ANG (1 7)-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ANG (1 7). Since the number of sites is limited, as more sites are occupied by ANG (1 7) from the sample, fewer sites are left to bind ANG (1 7)-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANG (1 7) concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Angiotensin (1-7) ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Ang(1-7) and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Angiotensin (1-7) ELISA kit

Summary of the Assay Procedure for Rat Angiotensin (1-7) ELISA kit

Citations of Rat Angiotensin (1-7) ELISA kit

E02A0206 has been referenced in the below publications:

Effect of Jianpi Yiqi Huoxue Decoction on SHR Releasing Vasoactive Substances by Regulating ACE2-Ang-(l-7)-MAS-AKT Signal Pathways Regulate Releasing Vasoactive Substances.


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