Rat Angiotensin 2 ELISA kit (E02A0204) - Shanghai BlueGene Biotech CO., LTD.

Specifications of Rat Angiotensin 2 ELISA kit

Product Information
Cat. No.	E02A0204
Product Name	Rat Angiotensin 2 ELISA kit
Species	Rat
Product Size	48 Tests / 96 Tests
Concentration	0.5-10 ng/mL
Sensitivity	0.1 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	0.5 ng/mL	1 vial
STANDARD C (0.5mL)	1.0 ng/mL	1 vial
STANDARD D (0.5mL)	2.5 ng/mL	1 vial
STANDARD E (0.5mL)	5.0 ng/mL	1 vial
STANDARD F (0.5mL)	10 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

Ang2 ELISA kit uses an anti-Ang2 antibody and an Ang2-HRP conjugate in a competitive enzyme immunoassay method. Ang2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Ang2 antibody binding site between Ang2 from samples and Ang2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Ang2. Since the number of sites is limited, as more sites are occupied by Ang2 from the sample, fewer sites are left to bind Ang2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Ang2 concentration in each sample is interpolated from this standard curve.

Quality Control on Rat Angiotensin 2 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between Ang2 and analogues was observed.

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Summary of the Assay Procedure for Rat Angiotensin 2 ELISA kit

Citations of Rat Angiotensin 2 ELISA kit

E02A0204 has been referenced in the below publications：

The effect of different dosing schedule of perindopril on circadian rhythm of urine rennin-angiotensin system in spontaneously hypertensive rats.

The therapeutic effect and mechanism of betaine and enalapril alone and combined use in the renal hypertensive rats.

芪参徤脾方对自发性高血压大鼠降压及血管保护作用的机制研究。

电针刺激“足三里”、“曲池”穴对自发性高血压大鼠血压和肾功能影响的机制研究。

The influence of recombinant human brain natriuretic peptide and furosemide on diuretic effect in rats with heart failure with reduced ejection fraction and its mechanism.

Study on the role of renin angiotensin system in pathogenesis of nonalcoholic fatty liver disease.

Influence of Jianpi Yiqi Huoxue Decoction on Vasomotor Factors Expression in Vascular Endothelium of Spontaneously Hypertensive Rats.

The Falconoid Luteolin Mitigates the Myocardial Inflammatory Response Induced by High-Carbohydrate/High-Fat Diet in Wistar Rats.

Yogurt Containing the Probacteria Lactobacillus acidophilus Combined with Natural Antioxidants Mitigates Doxorubicin-Induced Cardiomyopathy in Rats.

Sacubitril/Valsartan Decreases Atrial Fibrillation Susceptibility by Inhibiting Angiotensin II-Induced Atrial Fibrosis Through p-Smad2/3, p-JNK, and p-p38 Signaling Pathways.

Ibrutin ib-induced pulmonary angiotensin-converting enzyme activation promotes atrial ﬁbrillation in rats.