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  • bovine serum albumin elisa 1

BlueGene Biotech Rat Angiotensinogen ELISA kit (E02A0497)

E02A0497 Rat Angiotensinogen ELISA kit

The Rat Angiotensinogen ELISA kit can be used to identify samples from the rat species. Angiotensinogen can also be called AGT, ANHU, SERPINA8, hFLT1, angiotensinogen.

Products

Specifications of Rat Angiotensinogen ELISA kit

Product Information

Cat. No.

E02A0497

Product Name

Rat Angiotensinogen ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

250-5000 pg/mL

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

250 pg/mL

1 vial

STANDARD C (0.5mL)

500 pg/mL

1 vial

STANDARD D (0.5mL)

1000 pg/mL

1 vial

STANDARD E (0.5mL)

2500 pg/mL

1 vial

STANDARD F (0.5mL)

5000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

AGT ELISA kit uses an anti-AGT antibody and an AGT-HRP conjugate in a competitive enzyme immunoassay method. AGT-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-AGT antibody binding site between AGT from samples and AGT-HRP conjugate, the intensity of the color is inversely proportional to the concentration of AGT. Since the number of sites is limited, as more sites are occupied by AGT from the sample, fewer sites are left to bind AGT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGT concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Angiotensinogen ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between AGT and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Angiotensinogen ELISA kit

Summary of the Assay Procedure for Rat Angiotensinogen ELISA kit

Citations of Rat Angiotensinogen ELISA kit

E02A0497 has been referenced in the below publications:

The effect of different dosing schedule of perindopril on circadian rhythm of urine rennin-angiotensin system in spontaneously hypertensive rats.

Interplay between brain stem angiotensins and monocyte chemoattractant protein-1 as a novel mechanism for pressor response after ischemic stroke.


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