E02A0497 Rat Angiotensinogen ELISA kit
The Rat Angiotensinogen ELISA kit can be used to identify samples from the rat species. Angiotensinogen can also be called AGT, ANHU, SERPINA8, hFLT1, angiotensinogen.
Specifications of Rat Angiotensinogen ELISA kit
Product Information | |
Cat. No. | E02A0497 |
Product Name | Rat Angiotensinogen ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000 pg/mL |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 250 pg/mL | 1 vial |
STANDARD C (0.5mL) | 500 pg/mL | 1 vial |
STANDARD D (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD E (0.5mL) | 2500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 5000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
AGT ELISA kit uses an anti-AGT antibody and an AGT-HRP conjugate in a competitive enzyme immunoassay method. AGT-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-AGT antibody binding site between AGT from samples and AGT-HRP conjugate, the intensity of the color is inversely proportional to the concentration of AGT. Since the number of sites is limited, as more sites are occupied by AGT from the sample, fewer sites are left to bind AGT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGT concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Angiotensinogen ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between AGT and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Angiotensinogen ELISA kit
Citations of Rat Angiotensinogen ELISA kit
E02A0497 has been referenced in the below publications：
The effect of different dosing schedule of perindopril on circadian rhythm of urine rennin-angiotensin system in spontaneously hypertensive rats.
Interplay between brain stem angiotensins and monocyte chemoattractant protein-1 as a novel mechanism for pressor response after ischemic stroke.
