E02A3107 Rat Anti β2 Domain Containing type 1 Antibody ELISA Kit
The Rat Anti β2 Domain Containing type 1 Antibody ELISA Kit can be used to identify samples from the rat species. Interleukin can also be called AB2DAb.
Specifications of Rat Anti β2 Domain Containing type 1 Antibody ELISA Kit
Product Information | |
Cat. No. | E02A3107 |
Product Name | Rat Anti β2 Domain Containing type 1 Antibody ELISA Kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
AB2DAb ELISA kit uses an anti-AB2DAb antibody and an AB2DAb-HRP conjugate in a competitive enzyme immunoassay method. AB2DAb-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-AB2DAb antibody binding site between AB2DAb from samples and AB2DAb-HRP conjugate, the intensity of the color is inversely proportional to the concentration of AB2DAb. Since the number of sites is limited, as more sites are occupied by AB2DAb from the sample, fewer sites are left to bind AB2DAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AB2DAb concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Anti β2 Domain Containing type 1 Antibody ELISA Kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between AB2DAb and analogues was observed. | |
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Summary of the Assay Procedure for Rat Anti β2 Domain Containing type 1 Antibody ELISA Kit
Citations of Rat Anti β2 Domain Containing type 1 Antibody ELISA Kit
E02A3107 has been referenced in the below publications：
Experimental study on effect of hepatocyte growth factor on the survival rate of random ultralong skin flap on the rat back.
