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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Apoptosis stimulating of p53 protein 1 ELISA kit (E02A1946)

E02A1946 Rat Apoptosis stimulating of p53 protein 1 ELISA kit

The Rat Apoptosis stimulating of p53 protein 1 ELISA kit can be used to identify samples from the rat species. Apoptosis stimulating of p53 protein 1 can also be called PPP1R13B, ASPP1, p53BP2-like, p85, protein phosphatase 1 regulatory subunit 13B.

Products

Specifications of Rat Apoptosis stimulating of p53 protein 1 ELISA kit

Product Information

Cat. No.

E02A1946

Product Name

Rat Apoptosis stimulating of p53 protein 1 ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/ml

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

PPP1R13B ELISA kit uses an anti-PPP1R13B antibody and an PPP1R13B-HRP conjugate in a competitive enzyme immunoassay method. PPP1R13B-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PPP1R13B antibody binding site between PPP1R13B from samples and PPP1R13B-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PPP1R13B. Since the number of sites is limited, as more sites are occupied by PPP1R13B from the sample, fewer sites are left to bind PPP1R13B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PPP1R13B concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Apoptosis stimulating of p53 protein 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PPP1R13B and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Apoptosis stimulating of p53 protein 1 ELISA kit

Summary of the Assay Procedure for Rat Apoptosis stimulating of p53 protein 1 ELISA kit

Citations of Rat Apoptosis stimulating of p53 protein 1 ELISA kit

E02A1946 has been referenced in the below publications:

糖尿病大鼠视网膜p53、p21WAF1/CIP1表达研究。


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