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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Aquaporin 2, Collecting Duct ELISA kit (E02A0465)

E02A0465 Rat Aquaporin 2, Collecting Duct ELISA kit

The Rat Aquaporin 2, Collecting Duct ELISA kit can be used to identify samples from the rat species. Aquaporin 2, Collecting Duct can also be called AQP2, AQP-CD, WCH-CD, aquaporin 2, NDI2.

Products

Specifications of Rat Aquaporin 2, Collecting Duct ELISA kit

Product Information

Cat. No.

E02A0465

Product Name

Rat Aquaporin 2, Collecting Duct ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml

1 vial

STANDARD B (0.5mL)

2.5 ng/ml

1 vial

STANDARD C (0.5mL)

5.0 ng/ml

1 vial

STANDARD D (0.5mL)

10 ng/ml

1 vial

STANDARD E (0.5mL)

25 ng/ml

1 vial

STANDARD F (0.5mL)

50 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

AQP 2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AQP 2. Standards or samples are then added to the microtiter plate wells and AQP 2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AQP 2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AQP 2 are added to each well to “sandwich” the AQP 2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AQP 2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AQP 2 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Aquaporin 2, Collecting Duct ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between AQP 2 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Aquaporin 2, Collecting Duct ELISA kit

Summary of the Assay Procedure for Rat Aquaporin 2, Collecting Duct ELISA kit

Citations of Rat Aquaporin 2, Collecting Duct ELISA kit

E02A0465 has been referenced in the below publications:

Effect of Bushen Huoxue Decoction on Cardiac Function and Expression of AQP-2 in CHF Rats.

The influence of recombinant human brain natriuretic peptide and furosemide on diuretic effect in rats with heart failure with reduced ejection fraction and its mechanism.


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