E02A0465 Rat Aquaporin 2, Collecting Duct ELISA kit
The Rat Aquaporin 2, Collecting Duct ELISA kit can be used to identify samples from the rat species. Aquaporin 2, Collecting Duct can also be called AQP2, AQP-CD, WCH-CD, aquaporin 2, NDI2.
Specifications of Rat Aquaporin 2, Collecting Duct ELISA kit
Product Information | |
Cat. No. | E02A0465 |
Product Name | Rat Aquaporin 2, Collecting Duct ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/ml | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/ml | 1 vial |
STANDARD D (0.5mL) | 10 ng/ml | 1 vial |
STANDARD E (0.5mL) | 25 ng/ml | 1 vial |
STANDARD F (0.5mL) | 50 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
AQP 2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AQP 2. Standards or samples are then added to the microtiter plate wells and AQP 2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AQP 2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AQP 2 are added to each well to “sandwich” the AQP 2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AQP 2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AQP 2 concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Aquaporin 2, Collecting Duct ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between AQP 2 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Aquaporin 2, Collecting Duct ELISA kit
Citations of Rat Aquaporin 2, Collecting Duct ELISA kit
E02A0465 has been referenced in the below publications：
Effect of Bushen Huoxue Decoction on Cardiac Function and Expression of AQP-2 in CHF Rats.
The influence of recombinant human brain natriuretic peptide and furosemide on diuretic effect in rats with heart failure with reduced ejection fraction and its mechanism.
