E02F0003 Rat Basic Fibroblast growth factor ELISA kit
The Rat Basic Fibroblast growth factor ELISA kit can be used to identify samples from the rat species. Basic Fibroblast growth factor can also be called B-FGF, BFGF, FGFB, HBGH-2, Basic Fibroblast Growth Factor, Heparin-binding growth factor 2.
Product Information | |
Cat. No. | E02F0003 |
Product Name | Rat Basic Fibroblast growth factor ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500 pg/mL |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
bFGF ELISA kit uses an anti-bFGF antibody and an bFGF-HRP conjugate in a competitive enzyme immunoassay method. bFGF-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-bFGF antibody binding site between bFGF from samples and bFGF-HRP conjugate, the intensity of the color is inversely proportional to the concentration of bFGF. Since the number of sites is limited, as more sites are occupied by bFGF from the sample, fewer sites are left to bind bFGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The bFGF concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between bFGF and analogues was observed. | |
E02F0003 has been referenced in the below publications：
The Effect of Zhuang Jin Xu Gu Decoction in Promoting the Expression of CGRP and IGF-1 in Rats.
Comparison of two preparation methods of liver whole-organ decellularized matrix.
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