E02B1082 Rat bicinchoninic acid ELISA kit
The Rat bicinchoninic acid ELISA kit can be used to identify samples from the rat species. bicinchoninic acid can also be called Bicinchoninic acid;4,4′-Dicarboxy-2,2′-biquinoline;2,2'-Biquinoline-4,4'-dicarboxylic acid, BCA.
Product Information | |
Cat. No. | E02B1082 |
Product Name | Rat bicinchoninic acid ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ug/ml |
Sensitivity | 1.0 ug/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ug/mL | 1 vial |
STANDARD C (0.5mL) | 10 ug/mL | 1 vial |
STANDARD D (0.5mL) | 25 ug/mL | 1 vial |
STANDARD E (0.5mL) | 50 ug/mL | 1 vial |
STANDARD F (0.5mL) | 100 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
BCA ELISA kit uses an anti-BCA antibody and an BCA-HRP conjugate in a competitive enzyme immunoassay method. BCA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-BCA antibody binding site between BCA from samples and BCA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of BCA. Since the number of sites is limited, as more sites are occupied by BCA from the sample, fewer sites are left to bind BCA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BCA concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between BCA and analogues was observed. | |
E02B1082 has been referenced in the below publications：
The effects of platelet—-rich plasma Oll proliferative and osteogenic capabilities of cultured rat bone marrow mesenchymal stem ceils in vitr.
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