E02B0697 Rat Blood Urea Nitrogen ELISA kit
The Rat Blood Urea Nitrogen ELISA kit can be used to identify samples from the rat species. Blood Urea Nitrogen can also be called BUN.
Specifications of Rat Blood Urea Nitrogen ELISA kit
Product Information | |
Cat. No. | E02B0697 |
Product Name | Rat Blood Urea Nitrogen ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 mmol/L |
Sensitivity | 0.1 mmol/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 mmol/L | 1 vial |
STANDARD B (0.5mL) | 0.5 mmol/L | 1 vial |
STANDARD C (0.5mL) | 1.0 mmol/L | 1 vial |
STANDARD D (0.5mL) | 2.5 mmol/L | 1 vial |
STANDARD E (0.5mL) | 5.0 mmol/L | 1 vial |
STANDARD F (0.5mL) | 10 mmol/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
BUN ELISA kit uses an anti-BUN antibody and an BUN-HRP conjugate in a competitive enzyme immunoassay method. BUN-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-BUN antibody binding site between BUN from samples and BUN-HRP conjugate, the intensity of the color is inversely proportional to the concentration of BUN. Since the number of sites is limited, as more sites are occupied by BUN from the sample, fewer sites are left to bind BUN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BUN concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Blood Urea Nitrogen ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between BUN and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Blood Urea Nitrogen ELISA kit
Citations of Rat Blood Urea Nitrogen ELISA kit
E02B0697 has been referenced in the below publications：
Exoerim entalR esearch on Delaying Rats Chronic RenalFailure with Shenshuaining Pellet.
