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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Bone Gamma carboxyglutamic acid containing Proteins ELISA kit (E02B0137)

E02B0137 Rat Bone Gamma carboxyglutamic acid containing Proteins ELISA kit

The Rat Bone Gamma carboxyglutamic acid containing Proteins ELISA kit can be used to identify samples from the rat species. Bone Gamma carboxyglutamic acid containing Proteins can also be called Osteocalcin, BGLAP, BGP, OC, OCN.

Products

Specifications of Rat Bone Gamma carboxyglutamic acid containing Proteins ELISA kit

Product Information

Cat. No.

E02B0137

Product Name

Rat Bone Gamma carboxyglutamic acid containing Proteins ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/mL

Sensitivity

0.1ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SQSTM1 ELISA kit uses an anti-SQSTM1 antibody and an SQSTM1-HRP conjugate in a competitive enzyme immunoassay method. SQSTM1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SQSTM1 antibody binding site between SQSTM1 from samples and SQSTM1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SQSTM1. Since the number of sites is limited, as more sites are occupied by SQSTM1 from the sample, fewer sites are left to bind SQSTM1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SQSTM1 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Bone Gamma carboxyglutamic acid containing Proteins ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between BGLAP and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Bone Gamma carboxyglutamic acid containing Proteins ELISA kit

Summary of the Assay Procedure for Rat Bone Gamma carboxyglutamic acid containing Proteins ELISA kit

Citations of Rat Bone Gamma carboxyglutamic acid containing Proteins ELISA kit

E02B0137 has been referenced in the below publications:

Experimental Study of Herba Epimedii and Fructus Ligustri Lucid on the Glucocorticoid-induced Osteoporosis Rats based on regulation of TGF-beta 1/Smads signaling pathway.

Effects of Aluminum Exposure on the Bone Stimulatory Growth Factors in Rats.

Effects of the combined Herba Epimedii and Fructus Ligustri Lucidi on bone turnover and TGF-β1/Smads pathway in GIOP rats.


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