E02B0390 Rat Bone Morphogenetic Protein 7 ELISA kit
The Rat Bone Morphogenetic Protein 7 ELISA kit can be used to identify samples from the rat species. Bone Morphogenetic Protein 7 can also be called BMP7, OP-1, bone morphogenetic protein 7.
Specifications of Rat Bone Morphogenetic Protein 7 ELISA kit
Product Information | |
Cat. No. | E02B0390 |
Product Name | Rat Bone Morphogenetic Protein 7 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500 pg/mL |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
BMP7 ELISA kit uses an anti-BMP7 antibody and an BMP7-HRP conjugate in a competitive enzyme immunoassay method. BMP7-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-BMP7 antibody binding site between BMP7 from samples and BMP7-HRP conjugate, the intensity of the color is inversely proportional to the concentration of BMP7. Since the number of sites is limited, as more sites are occupied by BMP7 from the sample, fewer sites are left to bind BMP7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BMP7 concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Bone Morphogenetic Protein 7 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between BMP7 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Bone Morphogenetic Protein 7 ELISA kit
Citations of Rat Bone Morphogenetic Protein 7 ELISA kit
E02B0390 has been referenced in the below publications：
Experiment Research of Zhuang Jin Xu Gu Decoction in Promoting Fracture Healing Mechanism in Rats.
