En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Carcinoembryonic Antigen ELISA kit (E02C0348)

E02C0348 Rat Carcinoembryonic Antigen ELISA kit

The Rat Carcinoembryonic Antigen ELISA kit can be used to identify samples from the rat species. Carcinoembryonic Antigen can also be called CD66E; CD66; CEACAM5; Carcinoembryonic Antigen-related Cell Adhesion Molecule 5; Carcinoembryonic Antigen, CEA.

Products

Specifications of Rat Carcinoembryonic Antigen ELISA kit

Product Information

Cat. No.

E02C0348

Product Name

Rat Carcinoembryonic Antigen ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

10-250 ng/ml

Sensitivity

1.0 ng/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml

1 vial

STANDARD B (0.5mL)

10 ng/ml

1 vial

STANDARD C (0.5mL)

25 ng/ml

1 vial

STANDARD D (0.5mL)

50 ng/ml

1 vial

STANDARD E (0.5mL)

100 ng/ml

1 vial

STANDARD F (0.5mL)

250 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

CEA ELISA kit uses an anti-CEA antibody and an CEA-HRP conjugate in a competitive enzyme immunoassay method. CEA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CEA antibody binding site between CEA from samples and CEA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CEA. Since the number of sites is limited, as more sites are occupied by CEA from the sample, fewer sites are left to bind CEA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CEA concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Carcinoembryonic Antigen ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CEA and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Carcinoembryonic Antigen ELISA kit

Summary of the Assay Procedure for Rat Carcinoembryonic Antigen ELISA kit

Citations of Rat Carcinoembryonic Antigen ELISA kit

E02C0348 has been referenced in the below publications:

The Effects of Radon and Its Daughter on Targeted Organs and Protective Effect of Radon-protection agent in Rats.

Effects of radon on the gene methylation in BALF cells and tumor markers in serum with rats as experimental mode.


Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.