E02C0205 Rat Cartilage Oligomeric Matrix Protein ELISA kit
The Rat Cartilage Oligomeric Matrix Protein ELISA kit can be used to identify samples from the rat species. Cartilage Oligomeric Matrix Protein can also be called COMP, EDM1, EPD1, MED, PSACH, THBS5, cartilage oligomeric matrix protein, TSP5, CTS2.
Product Information | |
Cat. No. | E02C0205 |
Product Name | Rat Cartilage Oligomeric Matrix Protein ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 5-100 ng/mL |
Sensitivity | 1.0 ng/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/ml | 1 vial |
STANDARD C (0.5mL) | 10 ng/ml | 1 vial |
STANDARD D (0.5mL) | 25 ng/ml | 1 vial |
STANDARD E (0.5mL) | 50 ng/ml | 1 vial |
STANDARD F (0.5mL) | 100 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
COMP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for COMP. Standards or samples are then added to the microtiter plate wells and COMP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of COMP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for COMP are added to each well to “sandwich” the COMP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain COMP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COMP concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between COMP and analogues was observed. | |
E02C0205 has been referenced in the below publications：
Research and Development of a Compound Preparation for Treating Osteoarthritis.
Protective effect of S-adenosy-L-methionine combined with meloxicam on knee osteoarthritis in rats.
Related Bluegene Biotech Products
BlueGene Biotech News
