Rat Cathelicidin Antimicrobial Peptide ELISA kit (E02C0333)

BlueGene Biotech Rat Cathelicidin Antimicrobial Peptide ELISA kit (E02C0333)

E02C0333 Rat Cathelicidin Antimicrobial Peptide ELISA kit

The Rat Cathelicidin Antimicrobial Peptide ELISA kitcan be used to identify samples from the rat species. Cathelicidin Antimicrobial Peptide q can also be called Cathelicidin antimicrobial peptide, 18 kDa cationic antimicrobial protein, CAP-18, CAMP

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Specifications of Rat Cathelicidin Antimicrobial Peptide ELISA kit

Product Information
Cat. No.	E02C0333
Product Name	Rat Cathelicidin Antimicrobial Peptide ELISA kit
Species	Rat
Product Size	48 Tests / 96 Tests
Concentration	0.5-10 ng/ml
Sensitivity	0.1 ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	0.5 ng/mL	1 vial
STANDARD C (0.5mL)	1.0 ng/mL	1 vial
STANDARD D (0.5mL)	2.5 ng/mL	1 vial
STANDARD E (0.5mL)	5.0 ng/mL	1 vial
STANDARD F (0.5mL)	10 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

CAMP ELISA kit uses an anti-CAMP antibody and an CAMP-HRP conjugate in a competitive enzyme immunoassay method. CAMP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CAMP antibody binding site between CAMP from samples and CAMP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CAMP. Since the number of sites is limited, as more sites are occupied by CAMP from the sample, fewer sites are left to bind CAMP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAMP concentration in each sample is interpolated from this standard curve.

Quality Control on Rat Cathelicidin Antimicrobial Peptide ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between CAMP and analogues was observed.